HPLC Method for Analysis of Ketoconazole on Primesep B  Column

HPLC Method for Analysis of Ketoconazole on Primesep B by SIELC Technologies

HPLC Method for Analysis of Ketoconazole on Primesep B  Column by SIELC Technologies
Method for Analysis of Ketoconazole on Primesep B by SIELC Technologies

Ketoconazole, also known as Nizoral, is an antifungal compound with the chemical formula C26H28Cl2N4O4. It is used to treat dandruff and is commonly available as a shampoo. It is applied to skin to treat other fungal infections. Ketoconazole also has antiandrogen and antiglucocorticoid properties. It works through interfering with the fungal synthesis of ergosterol and other enzymes. You can find detailed UV spectra of Ketoconazole and information about its various lambda maxima by visiting the following link.

Ketoconazole can be retained and analyzed on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 15%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Peak Retention Time9.32

Description

Class of CompoundsDrug
Analyzing CompoundsKetoconazole

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Ketoconazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.