| CAS Number | 3844-45-9 |
|---|---|
| Molecular Formula | C37H34N2Na2O9S3 |
| Molecular Weight | 792.840 |
| InChI Key | SGHZXLIDFTYFHQ-UHFFFAOYSA-L |
| LogP | 2.09 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Brilliant Blue FCF Tartrazine, Sunset Yellow FCF and Allura Red in Monster Energy Drinks on Newcrom BH Column
July 14, 2020
Separation type: Liquid Chromatography Mixed-mode
Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden.
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain and separate dyes with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 400nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF and Allura Red
| Column | Newcom BH, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.75% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 400 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Brilliant Blue FCF, Allura Red, Tartrazine, Sunset Yellow FCF |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsBrilliant Blue FCF (Blue 1)
Sunset Yellow (Yellow 6)
Tartrazine (FD&C Yellow 5)
HPLC Method for Analysis of Brilliant Blue FCF and Allura Red in Monster Energy Ultra Violet, Sugar Free Energy Drink
July 2, 2020
Brilliant Blue FCF (Blue 1) is a synthetic molecule used as a food coloring agent. Allura Red (Red 40) is another food colorant. Both are present in Monster Energy Ultra Violet sugar free drink. Both are disodium salts and can be separated in HPLC by our Newcrom BH mixed-mode column which utilizes both hydrophobic and ion-exchange properties. The mobile phase is methanol (MeOH) and water with sulfuric acid (H2SO4) buffer making the method MS-compatible. Can also be UV detected at 210nm
| Column | Newcom BH, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.5%% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Brilliant Blue FCF, Allura Red |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsBrilliant Blue FCF (Blue 1)
HPLC Method for Analysis of Dyes in Walgreen’s Night Time Cough
March 29, 2017
| Column | Obelisc N, 3.26×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 630, 525 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red, Brilliant Blue |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsBrilliant Blue FCF (Blue 1)
