Brilliant Blue FCF (Blue 1)

Brilliant Blue structural formula

CAS Number3844-45-9
Molecular FormulaC37H34N2Na2O9S3
Molecular Weight792.840
InChI KeySGHZXLIDFTYFHQ-UHFFFAOYSA-L
LogP2.09
Synonyms
  • FD&C Blue No. 1
  • Disodium 2-{(Z)-(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)[(4Z)-4-{ethyl[(3-sulfonatophenyl)methyl]iminio}cyclohexa-2,5-dien-1-ylidene]methyl}benzene-1-sulfonate
  • Benzenemethanaminium, N-ethyl-N-[(4Z)-4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-, inner salt, sodium salt (1:2)
  • 3844-45-9
  • FD & C Blue No. 1 (Benzenemethanaminium, N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-, inner salt, disodium salt)
  • Benzenemethanaminium, N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-, inner salt, disodium salt
  • Benzenemethaminium, N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene-3-sulfo]-, hydroxide, inner salt, disodium salt
  • Benzenemethanaminium, N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-,inner salt, disodium salt
  • Benzenemethanaminium, N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-, hydroxide, inner salt, disodium salt
  • Benzenemethanaminium, N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-, inner salt, sodium salt (1:2)
  • Benzenemethanaminium, N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-,ydroxide, inner salt, disodium salt
  • Blue FCF
  • BRILLIANT BLUE FCF
  • C.I. ACID BLUE 9, DISODIUM SALT
  • C.I. FOOD BLUE
  • C.I. FOOD BLUE 2
  • CI 42090 sodium salt
  • dihidrogeno(etil)[4-[4-[etil(3-sulfonatobencil)]amino]-2'-sulfonatobencidriliden]ciclohexa-2,5-dien-1-iliden](3-sulfonatobencil)amonio, sal de disodio
  • dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)]amino]-2'-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt
  • Dihydrogen(ethyl)[4-[4-[ethyl(3-sulfonatobenzyl)]amino]-2'-sulfonatobenzhydryliden]cyclohexa-2,5-dien-1-yliden](3-sulfonatobenzyl)ammonium, Dinatriumsalz
  • Dihydrogeno(ethyl)[4-[4-[ethyl(3-sulfonatobenzyl)]amino]-2'-sulfonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulfonatobenzyl)ammonium, sel de disodium
  • dihydrogeno(ethyl)[4-[4-[ethyl(3-sulfonatobenzyl)]amino]-2'-sulfonatobenzhydrylidene]cyclohexa-2,5-diene-1-ylidene](3-sulfonatobenzyl)ammonium, sel de disodium
  • Disodium erioglaucine
  • FD AND C BLUE NO. 1
  • N-Ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl] (2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfobenzenemethanaminium hydroxide, inner salt, disodium salt
  • Aizen Food Blue No. 2
  • Brilliant Blue FCF Supra
  • Canacert Brilliant Blue FCF
  • Cosmetic Blue Lake
  • EINECS 223-339-8
  • FD & C Blue No. 1
  • FDC Blue No. 1
  • Fenazo Blue XI
  • Food Blue Dye No. 1
  • Hexacol Brilliant Blue A
  • Intracid Pure Blue L
  • Japan Blue 1
  • Japan Blue No. 1
  • Merantine Blue EG
  • Usacert Blue No. 1
  • Usacert FD and C Blue No. 1
  • Modr Brilantni FCF
  • Modr Kysela 9
  • Modr Potravinarska 2
  • UNII-H3R47K3TBD
  • FD&C Blue 1
  • Brilliant Blue E 133
  • D&C Blue No. 1
  • Brilliant Blue FCF, disodium salt
  • Brilliant Blue
  • 1314116-65-8
  • 145087-80-5
  • 511534-53-5
  • 70992-30-2
  • 73319-02-5
  • 82526-32-7
  • 83155-13-9
  • 1848225-75-1

Applications:

HPLC Method for Analysis of Brilliant Blue FCF Tartrazine, Sunset Yellow FCF and Allura Red in Monster Energy Drinks on Newcrom BH Column

July 14, 2020

Separation type: Liquid Chromatography Mixed-mode




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Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden.
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain and separate dyes with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 400nm.



High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF and Allura Red




 

Condition

Column Newcom BH, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 0.75%
Flow Rate 1.0 ml/min
Detection UV 400 nm

Description

Class of Compounds Acid, Dyes
Analyzing Compounds Brilliant Blue FCF, Allura Red, Tartrazine, Sunset Yellow FCF

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Allura Red (Red #40)
Brilliant Blue FCF (Blue 1)
Sunset Yellow (Yellow 6)
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Brilliant Blue FCF and Allura Red in Monster Energy Ultra Violet, Sugar Free Energy Drink

July 2, 2020


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Brilliant Blue FCF (Blue 1) is a synthetic molecule used as a food coloring agent. Allura Red (Red 40) is another food colorant. Both are present in Monster Energy Ultra Violet sugar free drink. Both are disodium salts and can be separated in HPLC by our Newcrom BH mixed-mode column which utilizes both hydrophobic and ion-exchange properties. The mobile phase is methanol (MeOH) and water with sulfuric acid (H2SO4) buffer making the method MS-compatible. Can also be UV detected at 210nm

Condition

Column Newcom BH, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 0.5%%
Flow Rate 1.0 ml/min
Detection UV 210 nm

Description

Class of Compounds Acid, Dyes
Analyzing Compounds Brilliant Blue FCF, Allura Red

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Allura Red (Red #40)
Brilliant Blue FCF (Blue 1)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Dyes in Walgreen’s Night Time Cough

March 29, 2017

 

Condition

Column Obelisc N, 3.26×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer AmFm pH 3.0 – 70 mM
Flow Rate 0.6 ml/min
Detection UV, 630, 525 nm

 

Description

Class of Compounds
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic
Analyzing Compounds Allura Red, Brilliant Blue

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Allura Red (Red #40)
Brilliant Blue FCF (Blue 1)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.