| CAS Number | 25956-17-6 |
|---|---|
| Molecular Formula | C18H14N2Na2O8S2 |
| Molecular Weight | 496.420 |
| InChI Key | CEZCCHQBSQPRMU-LLIZZRELSA-L |
| LogP | 1.56 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Allura Red (FD&C Red 40, E129) on a BIST A Column
November 30, 2022
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Allura Red (FD&C Red 40, E129)
Allura Red (FD&C Red 40, E129) is a popular red synthetic dye used in a wide variety of food products. Using SIELC’s newly introduced BIST™ method, Allura Red 1, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged Acid Red 1 analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Allura Red can be retained and UV detected at 500 nm.
Condition
| Column | BIST™ A, 4.6×150 mm, 5µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TMDAP phosphate – 5 mM pH 4.0 |
| Flow Rate | 2.0 mL/min |
| Detection | Vis 600 nm |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Allura Red (FD&C Red 40, E129) |
Application Column
BIST A
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
Select options
HPLC Method for Analysis of Brilliant Blue FCF Tartrazine, Sunset Yellow FCF and Allura Red in Monster Energy Drinks on Newcrom BH Column
July 14, 2020
Separation type: Liquid Chromatography Mixed-mode
Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden.
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain and separate dyes with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 400nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF and Allura Red
| Column | Newcom BH, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.75% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 400 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Brilliant Blue FCF, Allura Red, Tartrazine, Sunset Yellow FCF |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsBrilliant Blue FCF (Blue 1)
Sunset Yellow (Yellow 6)
Tartrazine (FD&C Yellow 5)
HPLC Method for Analysis of Brilliant Blue FCF and Allura Red in Monster Energy Ultra Violet, Sugar Free Energy Drink
July 2, 2020
Brilliant Blue FCF (Blue 1) is a synthetic molecule used as a food coloring agent. Allura Red (Red 40) is another food colorant. Both are present in Monster Energy Ultra Violet sugar free drink. Both are disodium salts and can be separated in HPLC by our Newcrom BH mixed-mode column which utilizes both hydrophobic and ion-exchange properties. The mobile phase is methanol (MeOH) and water with sulfuric acid (H2SO4) buffer making the method MS-compatible. Can also be UV detected at 210nm
| Column | Newcom BH, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.5%% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Brilliant Blue FCF, Allura Red |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsBrilliant Blue FCF (Blue 1)
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths
March 29, 2017
Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Obelisc N, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 430, 525 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red, Sunset Yellow, Tartrazine |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold
HPLC Method for Analysis of Allura Red in Robitussin Cough & Cold
HPLC Method for Analysis of Allura Red in Soft Drinks 2
HPLC Method for Analysis of Allura Red in Soft Drinks 3
HPLC Method for Analysis of Allura Red in Soft Drinks 4
HPLC Method for Analysis of Allura Red in Soft Drinks 5
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength
HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold
Sunset Yellow (Yellow 6)
Tartrazine (FD&C Yellow 5)
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength
March 29, 2017
Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Obelisc N, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 430 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red, Sunset Yellow, Tartrazine |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold
HPLC Method for Analysis of Allura Red in Robitussin Cough & Cold
HPLC Method for Analysis of Allura Red in Soft Drinks 2
HPLC Method for Analysis of Allura Red in Soft Drinks 3
HPLC Method for Analysis of Allura Red in Soft Drinks 4
HPLC Method for Analysis of Allura Red in Soft Drinks 5
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths
HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold
HPLC Method for Analysis of Dyes in Walgreen’s Night Time Cough
March 29, 2017
| Column | Obelisc N, 3.26×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 630, 525 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red, Brilliant Blue |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsBrilliant Blue FCF (Blue 1)
HPLC Method for Analysis of Allura Red in Robitussin Cough & Cold
March 29, 2017
Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Obelisc N, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 525 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold
HPLC Method for Analysis of Allura Red in Soft Drinks 2
HPLC Method for Analysis of Allura Red in Soft Drinks 3
HPLC Method for Analysis of Allura Red in Soft Drinks 4
HPLC Method for Analysis of Allura Red in Soft Drinks 5
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths
HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold
HPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold
March 29, 2017
Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Obelisc N, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 525 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Method for Analysis of Allura Red in Robitussin Cough & Cold
HPLC Method for Analysis of Allura Red in Soft Drinks 2
HPLC Method for Analysis of Allura Red in Soft Drinks 3
HPLC Method for Analysis of Allura Red in Soft Drinks 4
HPLC Method for Analysis of Allura Red in Soft Drinks 5
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths
HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold
HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold
March 29, 2017
Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. But Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Allura Red adn Brilliant Blue. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold
HPLC Method for Analysis of Allura Red in Robitussin Cough & Cold
HPLC Method for Analysis of Allura Red in Soft Drinks 2
HPLC Method for Analysis of Allura Red in Soft Drinks 3
HPLC Method for Analysis of Allura Red in Soft Drinks 4
HPLC Method for Analysis of Allura Red in Soft Drinks 5
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths
HPLC Method for Analysis of Allura Red in Sparkling Ice Cherry Limeade
March 29, 2017
HPLC Method for Analysis of Allura Red in Powerade Zero Fruit Punch
March 29, 2017
HPLC Method for Analysis of Allura Red in Soft Drinks 5
March 27, 2017
| Column | Obelisc N, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 525 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select options
HPLC Method for Analysis of Allura Red in Soft Drinks 3
March 27, 2017
| Column | Obelisc N, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 525 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select options
