HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold

Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. But Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Allura Red adn Brilliant Blue. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.