HPLC Method for Brilliant Blue FCF (Blue 1), Tartrazine (FD&C Yellow 5), Sunset Yellow (Yellow 6), Allura Red (Red #40) on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF (Blue 1), Tartrazine (FD&C Yellow 5), Sunset Yellow (Yellow 6), Allura Red (Red #40).
Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue.
Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.
Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden.
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns.
By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain and separate dyes with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 400nm.
You can find detailed UV spectra of Blue 1 and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Red 40 and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Yellow 6 and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Yellow 5 and information about its various lambda maxima by visiting the following link.
| Column | Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.75% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 400 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Brilliant Blue FCF (Blue 1), Tartrazine (FD&C Yellow 5), Sunset Yellow (Yellow 6), Allura Red (Red #40) |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Brilliant Blue FCF (Blue 1)
Sunset Yellow (Yellow 6)
Tartrazine (FD&C Yellow 5)
