HPLC Method for Analysis of Brilliant Blue FCF and Allura Red in Monster Energy Ultra Violet, Sugar Free Energy Drink

HPLC Method for Brilliant Blue FCF (Blue 1), Allura Red (Red #40) on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF (Blue 1), Allura Red (Red #40).

Brilliant Blue FCF (Blue 1) is a synthetic molecule used as a food coloring agent. Allura Red (Red 40) is another food colorant. Both are present in Monster Energy Ultra Violet sugar free drink. Both are disodium salts and can be separated in HPLC by our Newcrom BH mixed-mode column which utilizes both hydrophobic and ion-exchange properties. The mobile phase is methanol (MeOH) and water with sulfuric acid (H2SO4) buffer making the method MS-compatible. Can also be UV detected at 210nm

You can find detailed UV spectra of Blue 1 and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Red 40 and information about its various lambda maxima by visiting the following link.

Condition

Column Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 0.5%%
Flow Rate 1.0 ml/min
Detection UV 210 nm

Description

Class of Compounds Acid, Dyes
Analyzing Compounds Brilliant Blue FCF (Blue 1), Allura Red (Red #40)

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Allura Red (Red #40)
Brilliant Blue FCF (Blue 1)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.