HPLC Method for Analysis of Brilliant Blue FCF and Allura Red in Monster Energy Ultra Violet, Sugar Free Energy Drink

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Brilliant Blue FCF (Blue 1) is a synthetic molecule used as a food coloring agent. Allura Red (Red 40) is another food colorant. Both are present in Monster Energy Ultra Violet sugar free drink. Both are disodium salts and can be separated in HPLC by our Newcrom BH mixed-mode column which utilizes both hydrophobic and ion-exchange properties. The mobile phase is methanol (MeOH) and water with sulfuric acid (H2SO4) buffer making the method MS-compatible. Can also be UV detected at 210nm


Column Newcom BH, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 0.5%%
Flow Rate 1.0 ml/min
Detection UV 210 nm


Class of Compounds Acid, Dyes
Analyzing Compounds Brilliant Blue FCF, Allura Red


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Allura Red (Red #40)
Brilliant Blue FCF (Blue 1)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.