HPLC Determination of Erioglaucine on Newcrom BH Column

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Erioglaucine, more commonly known as Brilliant Blue FCF, is a synthetic coloring dye used in many industries as a colorant for foods, medications, cosmetics, etc. It is a multi-charged molecule and generally has poor peak shape on reverse-phase HPLC columns. By using a Newcrom BH mixed-mode column with both ion-exchange and hydrophobic properties, erioglaucine can be retained and exhibits great peak shape by using a mobile phase of methanol (MeOH) and water with sulfuric acid as buffer. UV detection at 625nm.


Column Newcom BH, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 50/50%
Buffer H2SO4 – 1%
Flow Rate 1.0 ml/min
Detection Vis  625  nm


Class of Compounds Acid, Dyes
Analyzing Compounds Erioglaucine


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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