HPLC Determination of Erioglaucine on Newcrom BH Column

HPLC.cloud View on hplc.cloud   Erioglaucine, more commonly known as Brilliant Blue FCF, is a synthetic coloring dye used in many industries as a colorant for foods, medications, cosmetics, etc. It is a multi-charged molecule and generally has poor peak shape on reverse-phase HPLC columns. By using a Newcrom BH mixed-mode column with both ion-exchange and hydrophobic properties, erioglaucine can be retained and exhibits great peak shape by using a mobile phase of methanol (MeOH) and water with sulfuric acid as buffer. UV detection at 625nm.


Column Newcom BH, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 50/50%
Buffer H2SO4 - 1%
Flow Rate 1.0 ml/min
Detection Vis  625  nm


Class of Compounds Acid, Dyes
Analyzing Compounds Erioglaucine
Application Analytes: