HPLC Method for Analysis of Brilliant Blue FCF (FD&C Blue No. 1) on Chromni Column

HPLC Method for Analysis of Brilliant Blue FCF (Blue 1) on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF (Blue 1).

Brilliant Blue FCF, also known as Blue 1, is a triarylmethane dye with the chemical formula C37H34N2Na2O9S3. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. It is used primarily as a blue colorant in medication, food, and cosmetics. It is one of the oldest FDA-approved color additives, having been listed since the 1969. You can find detailed UV spectra of Brilliant Blue FCF (Blue 1) and information about its various lambda maxima by visiting the following link.

Brilliant Blue FCF (Blue 1) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ammonium acetate. Detection is performed using UV.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O
BufferAmAc pH 5.0 – 10 mM
Flow Rate1.0 ml/min
DetectionVis 590 nm

Description

Class of CompoundsDyes
Analyzing CompoundsBrilliant Blue FCF (Blue 1)

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Brilliant Blue FCF (Blue 1)
Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.