Baclofen

Baclofen structural formula

CAS Number1134-47-0
Molecular FormulaC10H12ClNO2
Molecular Weight213.661
InChI KeyKPYSYYIEGFHWSV-UHFFFAOYSA-N
LogP-0.960
Synonyms
  • Baclofen
  • 4-Amino-3-(4-chlorophenyl)butanoic acid
  • Benzenepropanoic acid, beta-(aminomethyl)-4-chloro-
  • 1134-47-0
  • Benzenepropanoic acid, ?-(aminomethyl)-4-chloro-
  • (.+-.)-Baclofen
  • 4-Amino-3-(4-chlorophenyl)butyric acid
  • 4-Amino-3-(p-chlorophenyl)butyric acid
  • baclofene
  • baclofeno
  • CIBA Ba 34647
  • DL-4-Amino-3-p-chlorophenylbutanoic acid
  • dl-Baclofen
  • Hydrocinnamic acid, ?-(aminomethyl)-p-chloro-
  • Lioresal
  • ?-(4-Chlorophenyl)-?-aminobutyric acid
  • ?-(Aminomethyl)-4-chlorobenzenepropanoic acid
  • ?-(Aminomethyl)-p-chlorohydrocinnamic acid
  • ?-(p-Chlorophenyl)-?-aminobutyric acid
  • ?-p-Chlorophenyl-GABA
  • beta-(Aminomethyl)-p-chlorohydrocinnamic acid
  • BRN 2104494
  • Ciba 34,647-Ba
  • EINECS 214-486-9
  • (+-)-Baclofen
  • Lioresal Intrathecal
  • Kemstro
  • Gabalon
  • Baclofenum
  • UNII-H789N3FKE8
  • Gablofen
  • Atrofen
  • Apo-Baclofen
  • beta-(4-Chlorophenyl)gaba
  • beta-(Aminomethyl)-4-chlorobenzenepropanoic acid
  • beta-(Aminomethyl)-p-chlorohydrocinnamic acid
  • beta-(p-Chlorophenyl)-gamma-aminobutyric acid
  • gamma-Amino-beta-(p-chlorophenyl)butyric acid

Applications:

HPLC Method for Determination of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen on Primesep 100 Column

June 15, 2021

HPLC Method for gamma-Aminobutyric Acid (GABA), Baclofen, Pregabalin, Vigabatrin, Phenibut on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen

Gamma-Aminobutyric Acid (GABA) is a neurotransmitter with the chemical formula C4H9NO2. It is crucial in the nervous system for reducing anxiety and promoting sleep.

Vigabatrin is a medication with a chemical formula C6H11NO2. In Canada, it is approved for use as an adjunctive treatment for epilepsy, seizures, , and spasms in West syndrome.

Phenibut is a central nervous system (CNS) depressant with the chemical formula C10H13NO2. It is prescribed primarily in Russia and greater Eastern Europe to treat anxiety, insomnia, and other conditions.

Pregabalin is a medication with the chemical formula C8H17NO2. It is an anticonvulsant, analgesic, and anxiolytic amino acid that is used to treat epilepsy, restless leg syndrome, anxiety, neuropathic pain, and fibromyalgia. In the US it is used for adjunctive therapy for adults, while in the European Union, United Kingdom, and Russia, it is approved for treatment of generalized anxiety.

Baclofen is a central nervous system (CNS) depressant with the chemical formula C10H12ClNO2. It is used to treat spastic movement disorders. Off-balel, it is used as treatment for alcohol use disorder and opioid withdrawals.

All gabapentinoids can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 30/70%
BufferAmFm pH 3.0 – 50 mM
Flow Rate1.0  ml/min
DetectionCAD (Corona)  (MS-compatible mobile phase)
Class of Compounds
Acid, Drug
Analyzing Compoundsgamma-Aminobutyric Acid (GABA), Baclofen, Pregabalin, Vigabatrin, Phenibut

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Baclofen
Phenibut
Pregabalin
Vigabatrin
gamma-Aminobutyric Acid (GABA)
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Baclofen on Newcrom R1 HPLC column

February 16, 2018
Separation of Baclofen on Newcrom C18 HPLC column

Baclofen can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Baclofen
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.