If you are looking for optimized HPLC method to analyze Phenibut check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for gamma-Aminobutyric Acid (GABA), Baclofen, Pregabalin, Vigabatrin, Phenibut on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen

Gamma-Aminobutyric Acid (GABA) is a neurotransmitter with the chemical formula C₄H₉NO₂. It is crucial in the nervous system for reducing anxiety and promoting sleep.

Vigabatrin is a medication with a chemical formula C₆H₁₁NO₂. In Canada, it is approved for use as an adjunctive treatment for epilepsy, seizures, , and spasms in West syndrome.

Phenibut is a central nervous system (CNS) depressant with the chemical formula C₁₀H₁₃NO₂. It is prescribed primarily in Russia and greater Eastern Europe to treat anxiety, insomnia, and other conditions.

Pregabalin is a medication with the chemical formula C₈H₁₇NO₂. It is an anticonvulsant, analgesic, and anxiolytic amino acid that is used to treat epilepsy, restless leg syndrome, anxiety, neuropathic pain, and fibromyalgia. In the US it is used for adjunctive therapy for adults, while in the European Union, United Kingdom, and Russia, it is approved for treatment of generalized anxiety.

Baclofen is a central nervous system (CNS) depressant with the chemical formula C₁₀H₁₂ClNO₂. It is used to treat spastic movement disorders. Off-balel, it is used as treatment for alcohol use disorder and opioid withdrawals.

All gabapentinoids can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).