Baclofen

Baclofen structural formula

CAS Number1134-47-0
Molecular FormulaC10H12ClNO2
Molecular Weight213.661
InChI KeyKPYSYYIEGFHWSV-UHFFFAOYSA-N
LogP-0.960
Synonyms
  • Baclofen
  • 4-Amino-3-(4-chlorophenyl)butanoic acid
  • Benzenepropanoic acid, beta-(aminomethyl)-4-chloro-
  • 1134-47-0
  • Benzenepropanoic acid, ?-(aminomethyl)-4-chloro-
  • (.+-.)-Baclofen
  • 4-Amino-3-(4-chlorophenyl)butyric acid
  • 4-Amino-3-(p-chlorophenyl)butyric acid
  • baclofene
  • baclofeno
  • CIBA Ba 34647
  • DL-4-Amino-3-p-chlorophenylbutanoic acid
  • dl-Baclofen
  • Hydrocinnamic acid, ?-(aminomethyl)-p-chloro-
  • Lioresal
  • ?-(4-Chlorophenyl)-?-aminobutyric acid
  • ?-(Aminomethyl)-4-chlorobenzenepropanoic acid
  • ?-(Aminomethyl)-p-chlorohydrocinnamic acid
  • ?-(p-Chlorophenyl)-?-aminobutyric acid
  • ?-p-Chlorophenyl-GABA
  • beta-(Aminomethyl)-p-chlorohydrocinnamic acid
  • BRN 2104494
  • Ciba 34,647-Ba
  • EINECS 214-486-9
  • (+-)-Baclofen
  • Lioresal Intrathecal
  • Kemstro
  • Gabalon
  • Baclofenum
  • UNII-H789N3FKE8
  • Gablofen
  • Atrofen
  • Apo-Baclofen
  • beta-(4-Chlorophenyl)gaba
  • beta-(Aminomethyl)-4-chlorobenzenepropanoic acid
  • beta-(Aminomethyl)-p-chlorohydrocinnamic acid
  • beta-(p-Chlorophenyl)-gamma-aminobutyric acid
  • gamma-Amino-beta-(p-chlorophenyl)butyric acid

Applications:

HPLC Method for Determination of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen on Primesep 100 Column

June 15, 2021

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen

All gabapentinoids can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).

ColumnPrimesep 100,  4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 30/70%
BufferAmFm pH 3.0 – 50 mM
Flow Rate1.0  ml/min
DetectionCAD (Corona)  (MS-compatible mobile phase)
Class of Compounds
Acid, Drug
Analyzing CompoundsGamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Baclofen
Phenibut
Pregabalin
Vigabatrin
gamma-Aminobutyric Acid (GABA)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Baclofen on Newcrom R1 HPLC column

February 16, 2018
Separation of Baclofen on Newcrom C18 HPLC column

Baclofen can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Baclofen
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.