| CAS Number | 139755-83-2 |
|---|---|
| Molecular Formula | C22H30N6O4S |
| Molecular Weight | 474.580 |
| InChI Key | BNRNXUUZRGQAQC-UHFFFAOYSA-N |
| LogP | 2.75 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile Phase on Primesep 100 Column
December 12, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile Phase on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate (Viagra)
Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. Sildenafil can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be detected in the UV regime at 294 nm, and due to the Ammonium Formate buffer, is also compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 70% |
| Buffer | Ammonium Formate pH 3.0 – 60 mM |
| Flow Rate | 1.0 ml/min |
| Detection | λmax 294 nm |
| Peak Retention Time | 7.01 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Sildenafil Citrate (Viagra) |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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HPLC Method for Analysis of Sildenafil Citrate (Viagra) in a Tablet Primesep 100 Column
December 12, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil or Viagra on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil or Viagra
Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. Sildenafil can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 214 nm and 294 nm with high resolution and peak symmetry.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 60% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | λmax 214, 294 nm |
| Peak Retention Time | 5.7 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Sildenafil or Viagra |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Sildenafil on Newcrom R1 HPLC column
February 16, 2018
Sildenafil can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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