High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile Phase on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate (Viagra)
Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. Sildenafil can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be detected in the UV regime at 294 nm, and due to the Ammonium Formate buffer, is also compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 70% |
| Buffer | Ammonium Formate pH 3.0 – 60 mM |
| Flow Rate | 1.0 ml/min |
| Detection | λmax 294 nm |
| Peak Retention Time | 7.01 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Sildenafil Citrate (Viagra) |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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