HPLC Method for Sildenafil or Viagra on Primesep 100 by SIELC Technologies
Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. It works primarily through increasing bloodflow. It has the chemical formula C28H38N6O11S. It is a part of the group called phosphodiesterase 5 (PDE5) inhibitors, which prevent the enzyme called “phosphodiesterase type-5” from working.
Sildenafil or Viagra can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be detected in the UV regime at 294 nm, and due to the Ammonium Formate buffer, is also compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 70% |
| Buffer | Ammonium Formate pH 3.0 – 60 mM |
| Flow Rate | 1.0 ml/min |
| Detection | λmax 294 nm |
| Peak Retention Time | 7.01 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Sildenafil or Viagra |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
