HPLC Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile Phase on Primesep 100 Column

HPLC Method for Sildenafil or Viagra on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile on Primesep 100  Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil or Viagra.

Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension.

Sildenafil can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be detected in the UV regime at 294 nm, and due to the Ammonium Formate buffer, is also compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).

Condition

Column(Column variation not found)
Mobile PhaseMeCN – 70%
BufferAmmonium Formate pH 3.0 – 60 mM
Flow Rate1.0 ml/min
Detectionλmax 294 nm
Peak Retention Time7.01 min

Description

Class of CompoundsDrug
Analyzing CompoundsSildenafil or Viagra

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Sildenafil or Viagra
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.