Sildenafil or Viagra

Sildenafil structural formula

CAS Number139755-83-2
Molecular FormulaC22H30N6O4S
Molecular Weight474.580
InChI KeyBNRNXUUZRGQAQC-UHFFFAOYSA-N
LogP2.75
Synonyms
  • Sildenafil
  • 5-[2-Ethoxy-5-(4-methylpiperazine-1-sulfonyl)phenyl]-1-methyl-3-propyl-1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one
  • 7H-Pyrazolo[4,3-d]pyrimidin-7-one, 5-[2-ethoxy-5-[(4-methyl-1-piperazinyl)sulfonyl]phenyl]-1,6-dihydro-1-methyl-3-propyl-
  • 139755-83-2
  • Piperazine, 1-[[3-(4,7-dihydro-1-methyl-7-oxo-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-5-yl)-4-ethoxyphenyl]sulfonyl]-4-methyl-
  • 1-((3-(4,7-Dihydro-1-methyl-7-oxo-3-propyl-1H-pyrazolo(4,3-d)pyrimidin-5-yl)-4-ethoxyphenyl)sulfonyl)-4-methylpiperazine
  • UNII-3M7OB98Y7H
  • UK-92,480-10
  • 5-(2-ethoxy-5-(4-methylpiperazin-1-ylsulfonyl)phenyl)-1-methyl-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-7(6H)-one
  • 5-[2-Ethoxy-5-(4-methyl-piperazine-1-sulfonyl)-phenyl]-1-methyl-3-propyl-1,6-dihydro-pyrazolo[4,3-d]pyrimidin-7-one (Sildenafil or Viagra)
  • 5-[2-Ethoxy-5-(4-methyl-piperazine-1-sulfonyl)-phenyl]-1-methyl-3-propyl-1,6-dihydro-pyrazolo[4,3-d]pyrimidin-7-one(Sildenafil)

Applications:

HPLC Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile Phase on Primesep 100 Column

December 12, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile Phase on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Sildenafil Citrate (Viagra) MS-compatible Mobile on Primesep 100  Column
HPLC Method for Analysis of Sildenafil Citrate (Viagra) on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate (Viagra)

Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. Sildenafil can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be detected in the UV regime at 294 nm, and due to the Ammonium Formate buffer, is also compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 70%
BufferAmmonium Formate pH 3.0 – 60 mM
Flow Rate1.0 ml/min
Detectionλmax 294 nm
Peak Retention Time7.01 min

Description

Class of CompoundsDrug
Analyzing CompoundsSildenafil Citrate (Viagra)

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Sildenafil or Viagra
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Sildenafil Citrate (Viagra) in a Tablet Primesep 100 Column

December 12, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil or Viagra on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate on Primesep 100 by SIELC Technologies.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil or Viagra on Primesep 100 by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Sildenafil or Viagra

Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. Sildenafil can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 214 nm and 294 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 60%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
Detectionλmax 214, 294 nm
Peak Retention Time5.7 min

Description

Class of CompoundsDrug
Analyzing CompoundsSildenafil or Viagra

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Sildenafil or Viagra

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Sildenafil on Newcrom R1 HPLC column

February 16, 2018
Separation of Sildenafil on Newcrom C18 HPLC column

Sildenafil can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Sildenafil or Viagra
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.