Patulin

Patulin structural formula

CAS Number149-29-1
Molecular FormulaC7H6O4
Molecular Weight154.121
InChI KeyZRWPUFFVAOMMNM-UHFFFAOYSA-N
LogP-0.755
Synonyms
  • Patulin
  • 4-Hydroxy-4H-furo[3,2-c]pyran-2(6H)-one
  • 4H-Furo[3,2-c]pyran-2(6H)-one, 4-hydroxy-
  • 149-29-1
  • 4-Hydroxy-4H-furo[3,2c]pyran-2(6H)-one
  • 4H-Furo[3,2-c]pyran-2(6H)-one, 4-hydroxy-
  • 5-18-03-00005
  • 4,6-dihidro-4-hidroxi-2H-furo[3,2-c]piran-2-ona
  • 4,6-Dihydro-4-hydroxy-2H-furo[3,2-c]pyran-2-on
  • 4,6-dihydro-4-hydroxy-2H-furo[3,2-c]pyran-2-one
  • 4,6-dihydro-4-hydroxy-2H-furo[3,2-c]pyranne-2-one
  • 4-Hydroxy-4H-furo[3,2-c]pyran-2(6H)-one
  • Acetic acid, (2,4-dihydroxy-2H-pyran-3(6H)-ylidene)-, 3,4-lactone
  • Clairformin
  • Clavacin
  • Clavatin
  • Claviformin
  • DL-Patulin
  • Expansin
  • Expansine
  • Mycoin C
  • NSC 32951
  • NSC 8120
  • Patuline
  • Penicidin
  • Terinin
  • BRN 0149675
  • 2,4-Dihydroxy-2H-pyran-delta-3(6H),alpha-acetic acid-3,4-lactone
  • (2,4-Dihydroxy-2H-pyran-3(6H)-ylidene)acetic acid, 3,4-lactone
  • EINECS 205-735-2
  • 4H-Furo(3,3-c)pyran-2(6H)-one, 4-hydroxy-
  • 4-Hydroxy-4H-furo(3,2-c)pyran-2(6H)-one
  • Leucopin
  • Mycosin
  • 2H-Pyran-delta(sup 3(6H),alpha)-acetic acid, 2,4-dihydroxy-, 3,4-lactone
  • Tercinin
  • UNII-95X2BV4W8R
  • (2,4-dihydroxy-2H-pyran-3(6H)-ylidene)acetic acid-3,4-lactone
  • 4,6-dihydro-4-hydroxy-2H-furo(3,2-c)pyran-2-one
  • PAT
  • claviform
  • mycoin C3
  • mycoine C3

Applications:

HPLC Method for Analysis of Patulin on Newcrom R1 Column on Alltesta™

September 23, 2025

HPLC Method for Patulin on Newcrom R1 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Patulin

Patulin is a fungi produced mycotoxin with the molecular formula C7H6O4. It is lethal to rodents, as well as causing a myriad of health problems to other animals, including rats, mice, chickens, and hamsters. It is typically found on fruits and vegetables, especially apples. Extended exposure to patulin may potentially lead to tumor formation.

Patulin can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

ColumnNewcrom R1, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferPhosphoric Acid
Flow Rate0.5 ml/min
DetectionUV 272 nm

Class of CompoundsMycotoxin
Analyzing CompoundsPatulin

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Patulin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method For Analysis Of  Patulin on Newcrom R1 Column

August 19, 2021

Separation type: Liquid Chromatography Reverse Phase.


HPLC.cloud
View on hplc.cloud

High Performance Liquid Chromatography (HPLC) Method for Analysis of Patulin.

Patulin is a toxic chemical contaminant produced by several species of mold, especially within Byssochlamys Aspergillus and Penicillium. It is the most common mycotoxin found in apples and apple-derived products such as juice, cider, compotes and other food intended for young children. Patulin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN, ACN), water and Phosphoric Acid (H3PO4) buffer. The analysis method can be UV detected at 275 nm.

Condition
Column Newcrom R1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN – 10%
Buffer H3PO4 – 0.2%
Flow Rate 0.5 ml/min
UV Detection 275 nm
LOD 0.002 ppm

Description
Class of Compounds Hydrophilic
Analyzing Compounds Patulin

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Patulin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Patulin on Newcrom R1 HPLC column

February 16, 2018
Separation of Patulin on Newcrom C18 HPLC column

Patulin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Patulin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.