HPLC Method for Patulin on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Patulin
Patulin is a fungi produced mycotoxin with the molecular formula C7H6O4. It is lethal to rodents, as well as causing a myriad of health problems to other animals, including rats, mice, chickens, and hamsters. It is typically found on fruits and vegetables, especially apples. Extended exposure to patulin may potentially lead to tumor formation.
Patulin can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
Column | Newcrom R1, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN – 10% |
Buffer | Phosphoric Acid |
Flow Rate | 0.5 ml/min |
Detection | UV 272 nm |
Class of Compounds | Mycotoxin |
Analyzing Compounds | Patulin |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended