HPLC Method for Analysis of Patulin on Newcrom R1 Column on Alltesta™

HPLC Method for Patulin on Newcrom R1 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Patulin

Patulin is a fungi produced mycotoxin with the molecular formula C7H6O4. It is lethal to rodents, as well as causing a myriad of health problems to other animals, including rats, mice, chickens, and hamsters. It is typically found on fruits and vegetables, especially apples. Extended exposure to patulin may potentially lead to tumor formation.

Patulin can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

ColumnNewcrom R1, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferPhosphoric Acid
Flow Rate0.5 ml/min
DetectionUV 272 nm

Class of CompoundsMycotoxin
Analyzing CompoundsPatulin

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Patulin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.