HPLC Method for Separation of a Mixture of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 Column

HPLC Method for Analysis of Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-PhenylenediamineMobile PhaseMeCN/H2O – 30/70%BufferH2SO4 -0.2%Flow Rate1.0 ml/minDetectionUV 210 nmSamplesN,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL)
p-Phenylenediamine (1.3 mg/mL)
1-Naphthol (0.18 mg/mL)
Resorcinol (0.28 mg/mL)Injection volume3 µl

Class of Compounds
Phenols, Aromatic amines
Analyzing CompoundsResorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
1-Naphthol
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate
Resorcinol
p-Phenylenediamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.