| CAS Number | 108-46-3 |
|---|---|
| Molecular Formula | C6H6O2 |
| Molecular Weight | 110.112 |
| InChI Key | GHMLBKRAJCXXBS-UHFFFAOYSA-N |
| LogP | 0.8 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Resorcinol
January 28, 2026
If you are looking for optimized HPLC method to analyze Resorcinol check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Separation of a Mixture of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 Column
November 29, 2023
HPLC Method for Analysis of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Resorcinol, N,N-Bis(2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol
Resorcinol – a chemical compound with the formula C6H4(OH)2. It is a dihydroxybenzene used in various applications, including the production of resins and as a topical antiseptic.
N,N-Bis(2-hydroxyethyl)p-phenylenediamine – a compound used in the manufacture of hair dyes and related products.
p-Phenylenediamine – commonly known as PPD, it’s used in hair dyes and black rubber products.
1-Naphthol – a crystalline compound with the formula C10H7OH. It’s used in the synthesis of dyes and perfumes.
All compounds can be retained, separated, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL) p-Phenylenediamine (1.3 mg/mL) 1-Naphthol (0.18 mg/mL) Resorcinol (0.28 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Phenols, Aromatic amines |
| Analyzing Compounds | Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, m-Phenylenediamine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate
Resorcinol
m-Phenylenediamine
Separation of Resorcinol on Newcrom R1 HPLC column
February 16, 2018
Resorcinol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options