1-Naphthol structural formula

CAS Number90-15-3
Molecular FormulaC10H8O
Molecular Weight144.174
  • 1-Naphthol
  • Naphthalen-1-ol
  • 1-Naphthalenol
  • 90-15-3
  • 1-naphthalenol
  • 1-Naphthyl alcohol
  • BASF Ursol ERN
  • C.I. Oxidation Base 33
  • Durafur Developer D
  • Fouramine ERN
  • Fourrine 99
  • Fourrine ERN
  • Furro ER
  • Nako TRB
  • NAPHTHOL, ?-
  • NSC 9586
  • Oxidation Base 33
  • Tertral ERN
  • Ursol ERN
  • Zoba ERN
  • ?-Hydroxynaphthalene
  • ?-Naphthyl alcohol
  • CI Oxidation Base 33
  • EINECS 201-969-4
  • UNII-2A71EAQ389
  • Naphthol-1
  • Naphthyl-1-ol
  • Tertral ERN
  • alpha-Naphthol
  • alpha-Naphthyl alcohol
  • alpha-hydroxynaphthalene
  • 50356-21-3


HPLC Method for Separation of a Mixture of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 Column

November 29, 2023

HPLC Method for Analysis of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Resorcinol, N,N-Bis(2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphtholon Primesep 100 Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Resorcinol, N,N-Bis(2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol

Resorcinol – a chemical compound with the formula C6H4(OH)2. It is a dihydroxybenzene used in various applications, including the production of resins and as a topical antiseptic.

N,N-Bis(2-hydroxyethyl)p-phenylenediamine – a compound used in the manufacture of hair dyes and related products.

p-Phenylenediamine – commonly known as PPD, it’s used in hair dyes and black rubber products.

1-Naphthol – a crystalline compound with the formula C10H7OH. It’s used in the synthesis of dyes and perfumes.

All compounds can be retained, separated, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 30/70%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 210 nm
SamplesN,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL)
p-Phenylenediamine (1.3 mg/mL)
1-Naphthol (0.18 mg/mL)
Resorcinol (0.28 mg/mL)
Injection volume3 µl

Class of Compounds
Phenols, Aromatic amines
Analyzing CompoundsResorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 1-Naphthol on Newcrom R1 HPLC column

February 16, 2018
Separation of 1-Naphthol on Newcrom C18 HPLC column

1-Naphthol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.