HPLC Method for Separation of a Mixture of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 Column

HPLC Method for Analysis of Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine can be retained, separated, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4 -0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm
Samples N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL)
p-Phenylenediamine (1.3 mg/mL)
1-Naphthol (0.18 mg/mL)
Resorcinol (0.28 mg/mL)
Injection volume 3 µl
Class of Compounds
 Phenols, Aromatic amines
Analyzing Compounds Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
1-Naphthol
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate
Resorcinol
p-Phenylenediamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.