HPLC Method for Analysis of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Resorcinol, N,N-Bis(2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol
Resorcinol – a chemical compound with the formula C6H4(OH)2. It is a dihydroxybenzene used in various applications, including the production of resins and as a topical antiseptic.
N,N-Bis(2-hydroxyethyl)p-phenylenediamine – a compound used in the manufacture of hair dyes and related products.
p-Phenylenediamine – commonly known as PPD, it’s used in hair dyes and black rubber products.
1-Naphthol – a crystalline compound with the formula C10H7OH. It’s used in the synthesis of dyes and perfumes.
All compounds can be retained, separated, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL) p-Phenylenediamine (1.3 mg/mL) 1-Naphthol (0.18 mg/mL) Resorcinol (0.28 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Phenols, Aromatic amines |
| Analyzing Compounds | Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, m-Phenylenediamine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate
Resorcinol
m-Phenylenediamine
