
| CAS Number | 54381-16-7 |
|---|---|
| Molecular Formula | C10H18N2O6S |
| Molecular Weight | 294.33 |
| InChI Key | KMCFMEHSEWDYKG-UHFFFAOYSA-N |
| Synonyms |
|
Applications:
HPLC Method for Analysis of N,N-Bis (2-hydroxy)-p-phenylenediamine on Primesep 100 Column
November 29, 2023
HPLC Method for Analysis of N,N-Bis (2-hydroxy)-p-phenylenediamine on Primesep 100 by SIELC Technologies

N,N-Bis(2-hydroxyethyl)-p-phenylenediamine, often abbreviated as N,N-Bis(2-hydroxyethyl)-PPD, is a chemical compound with the chemical formula C10H18N2O6S. It is a derivative of p-phenylenediamine (PPD), which is a common ingredient in hair dyes.
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Aromatic amines |
| Analyzing Compounds | N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Separation of a Mixture of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 Column
November 29, 2023
HPLC Method for Analysis of Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine on Primesep 100 by SIELC Technologies
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL) p-Phenylenediamine (1.3 mg/mL) 1-Naphthol (0.18 mg/mL) Resorcinol (0.28 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds |
Phenols, Aromatic amines |
| Analyzing Compounds | Resorcinol, 1-Naphthol, N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate, p-Phenylenediamine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate
Resorcinol
p-Phenylenediamine
