HPLC Method for Analysis of N,N-Bis (2-hydroxy)-p-phenylenediamine on Primesep 100 Column

HPLC Method for Analysis of N,N-Bis (2-hydroxy)-p-phenylenediamine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of N,N-Bis(2-hydroxy)-p-phenylenediamine on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of N,N-Bis(2-hydroxy)-p-phenylenediamine

N,N-Bis(2-hydroxyethyl)-p-phenylenediamine (often abbreviated as N,N-Bis(2-hydroxyethyl)-PPD) is a chemical compound that is a derivative of p-phenylenediamine (PPD), a common ingredient in hair dyes.

The N,N-Bis(2-hydroxyethyl)-p-phenylenediamine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 30/70%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 210 nm
SamplesN,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL)
Injection volume3 µl

Class of Compounds
Aromatic amines
Analyzing CompoundsN,N-Bis(2-hydroxy)-p-phenylenediamine sulfate

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate
Application Detection:
UV Detection
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