HPLC Method for Analysis of N,N-Bis (2-hydroxy)-p-phenylenediamine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of N,N-Bis(2-hydroxy)-p-phenylenediamine
N,N-Bis(2-hydroxyethyl)-p-phenylenediamine (often abbreviated as N,N-Bis(2-hydroxyethyl)-PPD) is a chemical compound that is a derivative of p-phenylenediamine (PPD), a common ingredient in hair dyes.
The N,N-Bis(2-hydroxyethyl)-p-phenylenediamine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Aromatic amines |
| Analyzing Compounds | N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfateApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
