If you are looking for optimized HPLC method to analyze m-Phenylenediamine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

High Performance Liquid Chromatography (HPLC) Method for Analysis of o-Phenylenediamine, m-Phenylenediamine, p-Phenylenediamine on Primesep 100 by SIELC Technologies

o-Phenylenediamine, also written as OPD, is an isomer of phenylenediamine with the chemical formula C₆H₈N₂.It is used primarily as a precursor to heterocyclic compounds. It is condensed with a wide variety of compounds to produce various herbicides and pharmaceuticals. You can find detailed UV spectra of o-Phenylenediamine and information about its various lambda maxima by visiting the following link.

m-Phenylenediamine, also known as 1,3-diaminobenzene, is an isomer of phenylenediamine with the chemical formula C₆H₈N₂. It is used primarily in preparation of various polymers as well as in acceleration of adhesive resins. It is also a component of various dyes, including but not limited to various leather dyes, Basic Brown 1, Basic Orange 2, and Direct Black 38. You can find detailed UV spectra of m-Phenylenediamine and information about its various lambda maxima by visiting the following link.

p-Phenylenediamine, also written as PPD, is an isomer of phenylenediamine with the chemical formula C₆H₈N. IT is used primarily as a precursor to various polymers, a compound in hairdye, and as an antioxidant for rubber. You can find detailed UV spectra of p-Phenylenediamine and information about its various lambda maxima by visiting the following link.

These compounds are are isomers of phenylenediamine, where the amino groups are attached to different positions on the benzene ring. They are important in various industrial applications due to their role as intermediates in the synthesis of other chemicals.

o-Phenylenediamine, m-Phenylenediamine, p-Phenylenediamine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm