HPLC Method for Analysis of p-Phenylenediamine on Primesep 100 by SIELC Technologies
p-Phenylenediamine, commonly known as PPD, is an organic compound with the formula C6H8N2. used in hair dyes and black rubber products, especially kevlar and twaron.It’s use in hair dyes is being phased out, however, in favor of other aniline analogues. You can find detailed UV spectra of p-Phenylenediamine and information about its various lambda maxima by visiting the following link.
p-Phenylenediamine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | p-Phenylenediamine (1.3 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Aromatic amines |
| Analyzing Compounds | p-Phenylenediamine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
