HPLC Method for Analysis of p-Phenylenediamine on Primesep 100 Column

HPLC Method for Analysis of m-Phenylenediamine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of m-Phenylenediamine on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of m-Phenylenediamine

N,N-Bis(2-hydroxyethyl)-p-phenylenediamine (often abbreviated as N,N-Bis(2-hydroxyethyl)-PPD) is a chemical compound that is a derivative of p-phenylenediamine (PPD), a common ingredient in hair dyes.

The N,N-Bis(2-hydroxyethyl)-p-phenylenediamine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 30/70%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Samplesp-Phenylenediamine (1.3 mg/mL)
Injection volume3 µl

Class of Compounds
Aromatic amines
Analyzing Compoundsm-Phenylenediamine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
m-Phenylenediamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.