HPLC Method for Analysis of Isomers of Phenylenediamine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine

o-Phenylenediamine, m-Phenylenediamine, and p-Phenylenediamine are isomers of phenylenediamine, where the amino groups are attached to different positions on the benzene ring.

These compounds are important in various industrial applications due to their role as intermediates in the synthesis of other chemicals.

o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 40%
BufferH2SO4 -0.1%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Samples0.3 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*10 ppb (200 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Aromatic amines
Analyzing Compoundso-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

m-Phenylenediamine
o-Phenylenediamine
p-Phenylenediamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.