HPLC Determination of CBD, CBN, THC, 8-THC and THCA on Cannsep C Column

Separation type: Liquid Chromatography Reverse Phase.

HPLC Method for Analysis of Cannabidiol (CBD), Cannabinol (CBN), Tetrahydrocannabinol (THC), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC), Tetrahydrocannabinolic acid (THCA-A) on Cannsep C by SIELC Technologies

HPLC Determination of CBD, CBN, THC, 8-THC and THCA on Cannsep C Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, THC, 8-THC and THCA.

Δ8-THC, Δ9-THC, CBD, THCA and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH), Acetonitrile (MeCN, ACN), and water with sulfuric acid (H2SO4) as buffer and UV detected at 210nm.



ColumnCannsep C, 2.1 x 100 mm, 3 µm, 100 A
Mobile PhaseMeCN/MeOH/H2O – 45/40/15%
BufferH2SO4 – 0.2%
Flow Rate0.2 ml/min
DetectionUV, 200 nm
Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidiol (CBD),  Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC, Tetrahydrocannabinolic acid  (THCA)

Application Column

Cannsep C

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.