Sucrose

Sucrose structural formula

CAS Number57-50-1
Molecular FormulaC12H22O11
Molecular Weight342.297
InChI KeyCZMRCDWAGMRECN-UGDNZRGBSA-N
LogP-3.70
Synonyms
  • Sucrose
  • beta-D-Fructofuranosyl alpha-D-glucopyranoside
  • alpha-D-Glucopyranoside, beta-D-fructofuranosyl
  • 57-50-1
  • alpha-D-Glucopyranoside, beta-D-fructofuranosyl
  • D(+)-Sucrose
  • α-D-Glucopyranoside, β-D-fructofuranosyl
  • (+)-Sucrose
  • Amerfond
  • Beet sugar
  • Cane sugar
  • Carrare C 10
  • Confectioner's sugar
  • D-(+)-Saccharose
  • D-(+)-Sucrose
  • D-Sucrose
  • Frost Sugar
  • Granulated sugar
  • Khandsari
  • Manalox AS
  • Microse
  • NSC 406942
  • Rock candy
  • sacarosa
  • Saccharose
  • Saccharum
  • Sucralox
  • White sugar
  • α-D-GLUCOPYRANOSIDE, β-D- FRUCTOFURANOSYL
  • β-D-Fructofuranosyl α-D-glucopyranoside
  • β-D-Fructofuranosyl-α-D-glucopyranoside
  • Amerfand
  • EINECS 200-334-9
  • Fructofuranoside, alpha-D-glucopyranosyl, beta-D
  • beta-D-Fructofuranosyl alpha-D-glucopyranoside
  • Glucopyranoside, beta-D-fructofuranosyl, alpha-D
  • alpha-D-Glucopyranosyl beta-D-fructofuranoside
  • (alpha-D-Glucosido)-beta-D-fructofuranoside
  • NCI-C56597
  • Rohrzucker
  • Sucraloxum
  • UNII-C151H8M554
  • (2R,3R,4S,5R,6R)-2-[(2S,3S,4R,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
  • (2R,3R,4S,5R,6R)-2-[(2S,3S,4R,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl]oxy-6-(hydroxymethyl)tetrahydropyran-3,4,5-triol
  • (2R,3R,4S,5R,6R)-2-[(2S,3S,4R,5R)-3,4-dihydroxy-2,5-dimethylol-tetrahydrofuran-2-yl]oxy-6-methylol-tetrahydropyran-3,4,5-triol
  • (2R,3R,4S,5S,6R)-2-{[(2S,3S,4S,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy}-6-(hydroxymethyl)oxane-3,4,5-triol
  • Sacharose
  • White sugar
  • b -D-Fructofuranosyl a-D-glucopyranoside
  • beta-D-Fruf-(2<->1)-alpha-D-Glcp
  • table sugar
  • 100405-08-1
  • 104242-10-6
  • 1159795-78-4
  • 12040-73-2
  • 1206156-82-2
  • 131932-12-2
  • 146054-35-5
  • 146187-04-4
  • 151756-02-4
  • 220376-22-7
  • 29253-78-9
  • 29764-06-5
  • 30027-72-6
  • 47167-52-2
  • 47185-09-1
  • 47257-91-0
  • 50857-68-6
  • 51909-69-4
  • 635681-90-2
  • 64533-66-0
  • 65545-99-5
  • 75398-84-4
  • 76056-38-7
  • 78654-77-0
  • 786702-63-4
  • 80165-03-3
  • 8027-47-2
  • 8030-20-4
  • 85456-51-5
  • 86101-30-6
  • 87430-66-8
  • 880257-62-5
  • 92004-84-7

Applications:

HPLC Separation of Sugars on Primesep S2 Column

July 14, 2011

Five sugars were separated on a Primesep S2 HILIC column with LC/MS compatible conditions. Various mobile phase produce different selectivity and separation. Method can be used as alternative to aminopropyl column. Primesep S2 column is stable and does not undergo rapid hydrolysis like aminopropyl columns.

Condition

Column Primesep S2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0, Formic Acid
Flow Rate 1.0 ml/min
Detection ELSD 50C

 

Description

Class of Compounds
Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Fructose, Glucose, Sucrose, Lactose, Maltose, Sorbitol, Sucrose, Alfa D-Lactose, Beta D–Lactose, Raffinose

Application Column

Primesep S2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Fructose
Glucose
Lactose
Maltose
Raffinose
Sorbitol
Sucrose
alpha-D-Lactose
beta-D-Lactose

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80

July 8, 2011

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Barium ion and citric acid are analyzed in the presence of sucrose and Tween 80 on Obelisc N HILIC mixed-mode column. Both compounds are retained in ion-exchange mode and HILIC is not employed with this method. Obelisc N HPLC column has basic and acidic ion-exchange groups separated by long hydrophilic chain. Presence of that long chain allows both cation- and anion-exchange mechanisms to exist simultaneously. Obelisc N column can be used for analysis of cation and anions in HILIC/ion-exchange mode and in ion-exchange mode. Analytes can be monitored by ELSD/CAD and LC/MS.

 

Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD

 

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Description

Class of Compounds
 Acid, Ion,  Hydrophobic, Ionizable
Analyzing Compounds Barium ion, Citric acid,  Sucrose, Tween 80

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Citric Acid
Sucrose
Tween 80
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HILIC Separation of Sugars on Obelisc N

March 3, 2010


Common sugars can be separated on an Obelisc N HILIC/cation-exchange column with LC/MS compatible mobile phase. Highly polar surface of Obelisc N column provides good retention for sorbitol, sucrose, lactose and raffinose. Other sugars can be separated using this column.

Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Hydrophilic, Ionizable, Sugars
Analyzing Compounds Sorbitol, Sucrose, Lactose,  Raffinose

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Raffinose
Sorbitol
Sucrose
alpha-D-Lactose
beta-D-Lactose

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Effect of Both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids

March 3, 2010


In mixed-mode HILIC chromatography, selectivity of separation can be adjusted by amount of acetonitrile, amount of buffer and buffer pH. Buffer concentration and pH will affect retention of ionizable compounds to a different degree. Retention of neutral compounds can be adjusted by the amount of acetonitrile. Carboxylic acid, three amino acids and two sugars are separated by combination of HILIC and ion-exchange mechanisms. Compounds can be monitored by ELSD, Corona (CAD), LC/MS or low UV. UV-transparent mobile phase /buffer is required for UV monitoring of this mixed-mode separation. This HPLC method can be adopted as general approach for analysis of sugars, amino acids and carboxylic acids.

Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Succinic Acid, Phenylalanine, Sucrose, Glycine, Aspartic Acid, Raffinose

Application Column

Obelisc N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Aspartic Acid
Phenylalanine
Raffinose
Succinic Acid
Sucrose

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar

July 16, 2009

Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

Condition

Column Primesep D, 3.2×50 mm, 5 µm, 100A
Mobile Phase IPA
Buffer Formic Acid
Flow Rate 0.5 ml/min
Detection ELSD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Glycine, Histidine, Sucrose, Polysorbate 20

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amino Acids
Glycine
Histidine
Polysorbate
Polysorbate 20
Polysorbate 80
Sucrose
Tween 20
Tween 80

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.