5-Fluorouracil (5-FU, F5U)

5-Fluorouracil (5-FU, F5U) structural formula

CAS Number51-21-8
Molecular FormulaC4H3FN2O2
Molecular Weight130.079
InChI KeyGHASVSINZRGABV-UHFFFAOYSA-N
LogP-0.890
Synonyms
  • 5-Fluorouracil
  • 5-Fluoropyrimidine-2,4(1H,3H)-dione
  • 2,4(1H,3H)-Pyrimidinedione, 5-fluoro-
  • 51-21-8
  • 5-FU
  • Fluorouracil, 5-
  • 2,4(1H,3H)-Pyrimidinedione, 5-fluoro-
  • 2,4-Dihydroxy-5-fluoropyrimidine
  • 2,4-Dioxo-5-fluoropyrimidine
  • 5-Fluoracyl
  • 5-Fluoro-2,4(1H,3H)-pyrimidinedione
  • 5-Fluoro-2,4-pyrimidinedione
  • 5-Fluoropyrimidine-2,4-diol
  • 5-FLUORURACIL
  • Adrucil
  • Carzonal
  • Fluoroblastin
  • Fluoroplex
  • fluorouracil
  • Fluorouracile
  • fluorouracilo
  • Fluoruracil
  • Flurablastin
  • Fluracedyl
  • Fluracil
  • Fluracilum
  • Ftoruracil
  • Kecimeton
  • NSC 19893
  • Phthoruracil
  • Phtoruracil
  • Queroplex
  • Timazin
  • URACIL, 5-FLUORO-
  • Cinco FU
  • EINECS 200-085-6
  • 5-Fluoropyrimidin-2,4-diol
  • 5-Fluoropyrimidine-2,4-dione
  • Fluoro-uracile
  • Fluoro-uracilo
  • Fluorouracilum
  • Fluro uracil
  • Fluroblastin
  • NSC-19893
  • 5-Fluor-2,4(1H,3H)-pyrimidindion
  • 5-Fluor-2,4-dihydroxypyrimidin
  • 5-Fluor-2,4-pyrimidindiol
  • 5-Fluoracil
  • UNII-U3P01618RT
  • Fluorouracil Cream
  • 1004-03-1
  • 4921-97-5
  • 79108-01-3

Applications:

HPLC Method for Separation of Fluorouracil  and Flucytosine on Primesep 100 Column

January 30, 2023

HPLC Method for Separation of Fluorouracil  and Flucytosine on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Fluorouracil  and Flucytosineon Primesep 100 by SIELC Technologies
Method for Separation of Fluorouracil  and Flucytosine on Primesep 100 by SIELC Technologies

Fluorouracil (Adrucil) is a cytotoxic drug used intravenously in the treatment of a variety of cancers via chemotherapy, and as a topical medication for treating Actinic Keratosis and warts. Flucytosine (5-fluorocytosine, 5-FC) is a prescription-strength antifungal medication. Fluorouracil and Flucytosine can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 220 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Fluorouracil  and Flucytosine

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 1/99%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Peak Retention Time2.90, 6.81 min

Description

Class of CompoundsDrug
Analyzing CompoundsFluorouracil, Flucytosine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
5-Fluorouracil (5-FU, F5U)
Flucytosine (5-fluorocytosine, 5-FC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of 5-Fluorouracil on on Primesep 100 and Primesep P Columns with LC/MS Compatible Mobile Phase

July 16, 2009

5-Fluorouracil (5-FU) is pyrimidine analog which is used as anti-cancer drug. 5-Fluorouracil is very polar non-ionizable compound which is hard to retain by reversed-phase chromatography. Ion-pairing reagent cannot be employed due to non-ionizable nature of the compound. 5-fluorouracil is retained in zero organic on Primesep 100 and Primesep P columns. Both columns demonstrate perfect peak shape. 5-FU can be monitored by UV, or by ELSD, LC/MS or Corona CAD.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep P

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
5-Fluorouracil (5-FU, F5U)
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.