| CAS Number | 2022-85-7 |
|---|---|
| Molecular Formula | C4H4FN3O |
| Molecular Weight | 129.094 |
| InChI Key | XRECTZIEBJDKEO-UHFFFAOYSA-N |
| LogP | -1.18 |
| Synonyms |
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Applications:
HPLC Method for Separation of Fluorouracil and Flucytosine on Primesep 100 Column
January 30, 2023
HPLC Method for Separation of Fluorouracil and Flucytosine on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Fluorouracil (Adrucil) is a cytotoxic drug used intravenously in the treatment of a variety of cancers via chemotherapy, and as a topical medication for treating Actinic Keratosis and warts. Flucytosine (5-fluorocytosine, 5-FC) is a prescription-strength antifungal medication. Fluorouracil and Flucytosine can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 220 nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Fluorouracil and Flucytosine
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 1/99% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| Peak Retention Time | 2.90, 6.81 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Fluorouracil, Flucytosine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Flucytosine (5-fluorocytosine, 5-FC)
HPLC Method for Analysis of Flucytosine on Primesep 100 Column
January 27, 2023
HPLC Method for Analysis of Flucytosine on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
This antifungal medication can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Flucytosine
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 285 nm |
| Peak Retention Time | 4.81 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Flucytosine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Flucytosine on Newcrom R1 HPLC column
February 16, 2018
Flucytosine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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