| CAS Number | 51-21-8 |
|---|---|
| Molecular Formula | C4H3FN2O2 |
| Molecular Weight | 130.079 |
| InChI Key | GHASVSINZRGABV-UHFFFAOYSA-N |
| LogP | -0.890 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Fluorouracil
February 2, 2026
If you are looking for optimized HPLC method to analyze 5-Fluorouracil (5-FU, F5U) check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Separation of Fluorouracil and Flucytosine on Primesep 100 Column
January 30, 2023
HPLC Method for Separation of Fluorouracil and Flucytosine on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Fluorouracil (Adrucil) is a cytotoxic drug used intravenously in the treatment of a variety of cancers via chemotherapy, and as a topical medication for treating Actinic Keratosis and warts. Flucytosine (5-fluorocytosine, 5-FC) is a prescription-strength antifungal medication. Fluorouracil and Flucytosine can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 220 nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Fluorouracil and Flucytosine
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 1/99% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| Peak Retention Time | 2.90, 6.81 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Fluorouracil, Flucytosine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Flucytosine (5-fluorocytosine, 5-FC)
HPLC Analysis of 5-Fluorouracil on on Primesep 100 and Primesep P Columns with LC/MS Compatible Mobile Phase
July 16, 2009
5-Fluorouracil (5-FU) is pyrimidine analog which is used as anti-cancer drug. 5-Fluorouracil is very polar non-ionizable compound which is hard to retain by reversed-phase chromatography. Ion-pairing reagent cannot be employed due to non-ionizable nature of the compound. 5-fluorouracil is retained in zero organic on Primesep 100 and Primesep P columns. Both columns demonstrate perfect peak shape. 5-FU can be monitored by UV, or by ELSD, LC/MS or Corona CAD.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep P
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsUV Detection
