| CAS Number | 1912-24-9 |
|---|---|
| Molecular Formula | C8H14ClN5 |
| Molecular Weight | 215.691 |
| InChI Key | MXWJVTOOROXGIU-UHFFFAOYSA-N |
| LogP | 2.61 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Atrazine
February 19, 2026
Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Atrazine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Pesticides, Herbicides, Fungicides, and Insecticides on Newcrom BH Column
May 22, 2020
HPLC Method for Mecoprop, MCPB, Diuron, Simazine, Dicamba, Clopyralid, MCPA, Atrazine, Carbofuran, Fenvalerate, Folpet, Bentazone-sodium, 2,4,5-Trichlorophenoxyacetic acid, 4-CPA, Methyl parathion, Metribuzin, Monocrotophos on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mecoprop, MCPB, Diuron, Simazine, Dicamba, Clopyralid, MCPA, Atrazine, Carbofuran, Fenvalerate, Folpet, Bentazone-sodium, 2,4,5-Trichlorophenoxyacetic acid, 4-CPA, Methyl parathion, Metribuzin, Monocrotophos.
Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. All are heavily used in agriculture.
By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom BH mixed-mode column. It can be used with different organic media such as acetonitrile (ACN) or methanol (MeOH). By varying the concentration of organic modifier and using different buffers like sulfuric acid (H2SO4), ammonium formate (AmFm), or formic acid, separation of desired pesticides can be achieved.
| Column | Newcrom BH, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN, MeOH |
| Buffer | H2SO4, Formic acid, AmFm pH 3.0 |
| Flow Rate | 0.6 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Pesticides, Herbicides, Fungicides, Insecticides |
| Analyzing Compounds | Mecoprop, MCPB, Diuron, Simazine, Dicamba, Clopyralid, MCPA, Atrazine, Carbofuran, Fenvalerate, Folpet, Bentazone-sodium, 2,4,5-Trichlorophenoxyacetic acid, 4-CPA, Methyl parathion, Metribuzin, Monocrotophos |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
4-CPA
Atrazine
Bentazone-sodium
Carbofuran
Clopyralid
Dicamba
Diuron
Fenvalerate
Folpet
MCPA
MCPB
Mecoprop
Methyl parathion
Metribuzin
Monocrotophos
Simazine
Separation of Atrazine on Newcrom R1 HPLC column
May 16, 2018
Atrazine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options



