Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
DeoxyadenosineDeoxycytidine
Deoxyguanosine
Deoxyuridine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
