Zeaxanthin

Zeaxanthin structural formula

CAS Number144-68-3
Molecular FormulaC40H56O2
Molecular Weight568.886
InChI KeyJKQXZKUSFCKOGQ-QAYBQHTQSA-N
LogP10.9
Synonyms
  • Zeaxanthin
  • (3R,3'R)-beta,beta-Carotene-3,3'-diol
  • beta,beta-Carotene-3,3'-diol, (3R,3'R)-
  • 144-68-3
  • ?,?-Carotene-3,3'-diol, (3R,3'R)-
  • (3R,3'R)-Zeaxanthin
  • (3R,3'R)-?,?-carotene-3,3'-diol
  • (3R,3'R)-?,?-caroteno-3,3'-diol
  • (3R,3'R)-?,?-Carotin-3,3'-diol
  • all-trans-Anchovyxanthin
  • all-trans-Zeaxanthin
  • Anchovyxanthin
  • Anchovyxanthin, all-trans-
  • Xanthophyll 3
  • ZeaGold
  • Zeaxanthin, all-trans-
  • Zeaxanthol
  • ?-Carotene-3,3'-diol, (3R,3'R)-all-trans-
  • EINECS 205-636-4
  • 3R 3'R Zeaxanthin
  • 3R,3'R-Zeaxanthin
  • UNII-CV0IB81ORO
  • meso-Zeaxanthin
  • beta-Carotene-3,3'-diol
  • (1R)-4-[(1E,3E,5E,7E,9E,11E,13E,15E,17E)-18-[(4R)-4-hydroxy-2,6,6-trimethylcyclohex-1-en-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaen-1-yl]-3,5,5-trimethylcyclohex-3-en-1-ol
  • (3R,3'R)-Zeaxanthin
  • (3R,3'R)-dihydroxy-beta,beta-carotene
  • all-trans-beta-carotene-3,3'-diol
  • beta,beta-carotene-3,3'-diol

Applications:

HPLC MS Method for Analysis of Lutein and Zeaxanthin in Supplements on Primesep B Column

August 15, 2024

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of Lutein, Zeaxanthin on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Lutein, Zeaxanthin on Primesep B Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Lutein, Zeaxanthin

Lutein is an oxygenated carotenoid, or xanthophyll, with the chemical formula C₄₀H₅₆O₂. It has a chain of conjugated double bonds and hydroxyl groups at each end, which give it strong antioxidant properties. Lutein’s ability to absorb blue light helps protect the eyes from oxidative stress. In plants, lutein stabilizes chlorophyll and protects cells from light-induced damage.

Zeaxanthin is also a xanthophyll carotenoid, sharing the same chemical formula as lutein, C₄₀H₅₆O₂, but with a different arrangement of double bonds. This structural variation allows zeaxanthin to be particularly effective in protecting the central macula of the retina. It filters harmful blue light and shields the eyes from oxidative damage, similar to lutein.

Lutein, Zeaxanthin can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using Vis 450 nm.

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 85%
BufferFormic Acid -0.1%
Flow Rate1.0 ml/min
DetectionVis 450 nm, ESI MS SIM 551 +, 568+
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
xanthophylls
Analyzing CompoundsLutein, Zeaxanthin

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Lutein
Zeaxanthin

Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Lutein and Zeaxanthin in Supplements on Primesep B Column

August 13, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Lutein, Zeaxanthin on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Lutein, Zeaxanthin on Primesep B Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Lutein, Zeaxanthin

Lutein is an oxygenated carotenoid, or xanthophyll, with the chemical formula C₄₀H₅₆O₂. It has a chain of conjugated double bonds and hydroxyl groups at each end, which give it strong antioxidant properties. Lutein’s ability to absorb blue light helps protect the eyes from oxidative stress. In plants, lutein stabilizes chlorophyll and protects cells from light-induced damage.

Zeaxanthin is also a xanthophyll carotenoid, sharing the same chemical formula as lutein, C₄₀H₅₆O₂, but with a different arrangement of double bonds. This structural variation allows zeaxanthin to be particularly effective in protecting the central macula of the retina. It filters harmful blue light and shields the eyes from oxidative damage, similar to lutein.

Lutein, Zeaxanthin can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using Vis 450 nm.

ColumnPrimesep B, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseMeCN – 85%
BufferH2SO4 -0.1%
Flow Rate0.7 ml/min
DetectionVis 450 nm
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
xanthophylls
Analyzing CompoundsLutein, Zeaxanthin

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Lutein
Zeaxanthin

Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Zeaxanthin on Newcrom R1 HPLC column

February 16, 2018
Separation of Zeaxanthin on Newcrom C18 HPLC column

Zeaxanthin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Zeaxanthin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.