| CAS Number | 124832-26-4 |
|---|---|
| Molecular Formula | C13H20N6O4 |
| Molecular Weight | 324.342 |
| InChI Key | HDOVUKNUBWVHOX-QMMMGPOBSA-N |
| LogP | -0.521 |
| Synonyms |
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Applications:
HPLC Separation of Antiviral Drugs on Primesep 100 Column
April 1, 2021
HPLC Method for Acyclovir, Valacyclovir, Oseltamivir on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Valacyclovir, Oseltamivir.
Unlike antibiotic drugs, which actively destroy their targets, antiviral drugs generally prevent the reproduction of their viral targets. Acyclovir, Valacyclovir, and Oseltamivir are 3 antiviral drugs commonly prescribed to treat viral infections. Acyclovir and Valacyclovir are used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. They can also be used to treat chickenpox in children. Oseltamivir is generally used to help treat (or if taken early enough, prevent) influenza (the flu) infections.
Acyclovir, Valacyclovir, Oseltamivir can be retained on a Primesep 100 mixed-mode column with great peak shape using acetonitrile (ACN), water and a gradient of sulphuric acid (H2SO4) as a buffer. This method can be UV detected at 200 nm with very high resolution.
| Column | Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | Gradient H2SO4 – 0.05- 0.8%, 10 min |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 255 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Acyclovir, Valacyclovir, Oseltamivir |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Oseltamivir
Valacyclovir
Vis Detection
HPLC Determination of Valacyclovir on Primesep 100 Column
March 31, 2021
HPLC Method for Valacyclovir on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Valacyclovir
Valacyclovir belongs to a class of drugs called antiviral drugs and has the chemical formula C13H20N6O4. Valacyclovir is used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes as well as shingles. It can also be used to treat chickenpox in children.
Valacyclovir can be retained on a Valacyclovir mixed-mode column with great peak shape using an isocratic method of acetonitrile (ACN), water and sulfuric acid (H2SO4) as a buffer. UV Detection 255nm.
| Column | Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 255 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Valacyclovir |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Valacyclovir on Newcrom R1 HPLC column
February 16, 2018
Valacyclovir can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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