HPLC Separation of Antiviral Drugs on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Oseltamivir, Valacyclovir
Unlike antibiotic drugs, which actively destroy their targets, antiviral drugs generally prevent the reproduction of their viral targets. Acyclovir, Valacyclovir, and Oseltamivir are 3 antiviral drugs commonly prescribed to treat viral infections. Acyclovir and Valacyclovir are used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. They can also be used to treat chickenpox in children. Oseltamivir is generally used to help treat (or if taken early enough, prevent) influenza (the flu) infections. Acyclovir, Valacyclovir, and Oseltamivir can be retained on a Primesep 100 mixed-mode column with great peak shape using acetonitrile (ACN), water and a gradient of sulphuric acid (H2SO4) as a buffer. This method can be UV detected at 200 nm with very high resolution.


Column Primesep 100, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer Gradient H2SO4 - 0.05- 0.8%, 10 min
Flow Rate 0.5 ml/min
Detection UV, 255 nm


Class of Compounds Drug
Analyzing Compounds Acyclovir, Valacyclovir, Oseltamivir
Application Analytes: