| CAS Number | 738-70-5 |
|---|---|
| Molecular Formula | C14H18N4O3 |
| Molecular Weight | 290.323 |
| InChI Key | IEDVJHCEMCRBQM-UHFFFAOYSA-N |
| LogP | 0.91 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Trimethoprim
January 14, 2026
If you are looking for optimized HPLC method to analyze Trimethoprim check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method For Analysis Of Sulfamethoxazole and Trimethoprim on Primesep 100 Column
March 22, 2022
HPLC Method for Trimethoprim, Sulfamethoxazole on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimethoprim, Sulfamethoxazole.
Sulfamethoxazole, also written as SMZ and SMX, is an antibiotic with the chemical formula C10H11N3O3S. It is used primarily to treat bacterial infections including but not limited to bronchitis, prostatitis, urinary tract infections, as well as gam negative and positive bacteria. You can find detailed UV spectra of Sulfamethoxazole and information about its various lambda maxima by visiting the following link.
Trimethoprim, also written as TMP, is an antibiotic with the chemical formula C14H18N4O3. It is used primarily to treat bladder infections as well as middle ear infections and travelers’ diarrhea. People with HIV/AIDS might take it to treat Pneumocystis pneumonia. It works through binding to dihydrofolate reductase and inhibiting the production of dihydrofolic and tetrahydrofolic acid. You can find detailed UV spectra of Trimethoprim and information about its various lambda maxima by visiting the following link.
Trimethoprim, Sulfamethoxazole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm, |
| Class of Compounds |
Drug |
| Analyzing Compounds | Trimethoprim, Sulfamethoxazole |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Trimethoprim
Separation of Trimethoprim on Newcrom R1 HPLC column
February 16, 2018
Trimethoprim can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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