HPLC Method For Analysis Of Sulfamethoxazole and Trimethoprim on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode


HPLC.cloud
View on hplc.cloud

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfamethoxazole and Trimethoprim

Condition
Column Primesep 100, Newcrom R1 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer H2SO4 – 0.4%
Flow Rate 1.0 ml/min
Detection UV, 200 nm,

 

Description
Class of Compounds
 Drug
Analyzing Compounds Sulfamethoxazole, Trimethoprim

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:

Sulfamethoxazole
Trimethoprim
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.