HPLC Method for Trimethoprim, Sulfamethoxazole on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimethoprim, Sulfamethoxazole.
Sulfamethoxazole, also written as SMZ and SMX, is an antibiotic with the chemical formula C10H11N3O3S. It is used primarily to treat bacterial infections including but not limited to bronchitis, prostatitis, urinary tract infections, as well as gam negative and positive bacteria. You can find detailed UV spectra of Sulfamethoxazole and information about its various lambda maxima by visiting the following link.
Trimethoprim, also written as TMP, is an antibiotic with the chemical formula C14H18N4O3. It is used primarily to treat bladder infections as well as middle ear infections and travelers’ diarrhea. People with HIV/AIDS might take it to treat Pneumocystis pneumonia. It works through binding to dihydrofolate reductase and inhibiting the production of dihydrofolic and tetrahydrofolic acid. You can find detailed UV spectra of Trimethoprim and information about its various lambda maxima by visiting the following link.
Trimethoprim, Sulfamethoxazole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm, |
| Class of Compounds |
Drug |
| Analyzing Compounds | Trimethoprim, Sulfamethoxazole |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Trimethoprim
