| CAS Number | 3366-95-8 |
|---|---|
| Molecular Formula | C7H11N3O3 |
| Molecular Weight | 185.183 |
| InChI Key | KPQZUUQMTUIKBP-UHFFFAOYSA-N |
| LogP | 0.22 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Mixture of Nitroimidazole Antibiotics on Newcrom Type Columns
April 26, 2023
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
HPLC Method for Analysis of Secnidazole, Tinidazole, Metronidazole on Newcrom AH and Newcrom R1 Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Secnidazole, Tinidazole, Metronidazole
Tinidazole, metronidazole, and secnidazole are all nitroimidazole antibiotics used to treat bacterial and parasitic infections.
Metronidazole is the most commonly prescribed among the three and is used to treat a wide range of infections, including bacterial vaginosis, trichomoniasis, and certain types of anaerobic bacterial infections.
Tinidazole is similar to metronidazole in its mechanism of action and is used to treat similar infections, but it has a longer half-life and can be administered less frequently.
Secnidazole is a newer nitroimidazole antibiotic with a longer half-life than metronidazole, which allows for a shorter treatment duration. It is used to treat trichomoniasis and certain types of anaerobic bacterial infections.
It is important to note that these antibiotics can have side effects such as nausea, vomiting, and diarrhea, and should only be used as directed by a healthcare professional. Additionally, alcohol should be avoided while taking these medications as they can cause a severe reaction.
used to treat bacterial and parasitic infections.
Metronidazole is the most commonly prescribed among the three and is used to treat a wide range of infections, including bacterial vaginosis, trichomoniasis, and certain types of anaerobic bacterial infections.
Tinidazole is similar to metronidazole in its mechanism of action and is used to treat similar infections, but it has a longer half-life and can be administered less frequently.
Secnidazole is a newer nitroimidazole antibiotic with a longer half-life than metronidazole, which allows for a shorter treatment duration. It is used to treat trichomoniasis and certain types of anaerobic bacterial infections.
It is important to note that these antibiotics can have side effects such as nausea, vomiting, and diarrhea, and should only be used as directed by a healthcare professional. Additionally, alcohol should be avoided while taking these medications as they can cause a severe reaction.
These 3 nitroimidazole antibiotics can be separated and analyzed on a Newcrom AH (mixed-mode) and Newcrom R1 (reverse-phase) columns using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN, ACN), and phosphoric acid buffer. The analysis method can be UV detected at 270 nm with high resolution and peak symmetry.
Condition
| Column | Newcrom AH, 3.2 x 100 mm, 5 µm, 100 A, Newcrom R1 |
| Mobile Phase | MeCN – 20% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 270 nm |
Description
| Class of Compounds | Nitroimidazole antibiotics |
| Analyzing Compounds | Secnidazole, Tinidazole, Metronidazole |
Application Column
Newcrom AH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Secnidazole
Tinidazole
Separation of Secnidazole on Newcrom R1 HPLC column
February 16, 2018
Secnidazole can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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