| CAS Number | 16088-19-0 |
|---|---|
| Molecular Formula | C11H16N2O2 |
| Molecular Weight | 208.262 |
| InChI Key | FWNHTEHWJKUVPG-UHFFFAOYSA-N |
| LogP | 1.93 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Neostigmine
February 13, 2026
If you are looking for optimized HPLC method to analyze Norneostigmine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Neostigmine methylsulfate on Primesep 100 Column
May 11, 2022
HPLC Method for Neostigmine methylsulfate, Norneostigmine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Neostigmine methylsulfate, Norneostigmine.
Neostigmine methylsulfate is a drug with the chemical formula C13H22N2O6S. It is primarily used to reverse effects of non-depolarizing neuromuscular blocking agents after surgery. It works through inhibiting the enzyme acetylcholinesterase, preventing the breakdown of the neurotransmitter acetylcholine, which leads to a buildup of it at nerve endings, which stimulates muscle contractions.
Neostigmine methylsulfate, Norneostigmine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 30% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Neostigmine methylsulfate, Norneostigmine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Norneostigmine
Separation of Norneostigmine on Newcrom R1 HPLC column
May 16, 2018
Norneostigmine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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