CAS Number16088-19-0
Molecular FormulaC11H16N2O2
Molecular Weight208.262
  • Norneostigmine
  • 3-(Dimethylamino)phenyl dimethylcarbamate
  • Carbamic acid, N,N-dimethyl-, 3-(dimethylamino)phenyl ester
  • 16088-19-0
  • EINECS 240-240-5


HPLC Method for Analysis of Neostigmine methylsulfate on Primesep 100 Column

May 11, 2022

Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Neostigmine methylsulfate


Column Promesep 100, 4.6×150 mm,  100A
Mobile Phase MeCN – 30%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 210 nm


Class of Compounds Drug
Analyzing Compounds Neostigmine methylsulfate


Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Neostigmine methylsulfate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.

Separation of Norneostigmine on Newcrom R1 HPLC column

May 16, 2018
Separation of Norneostigmine on Newcrom R1 HPLC column

Norneostigmine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: or by phone: 847-229-2629.