CAS Number16088-19-0
Molecular FormulaC11H16N2O2
Molecular Weight208.262
  • Norneostigmine
  • 3-(Dimethylamino)phenyl dimethylcarbamate
  • Carbamic acid, N,N-dimethyl-, 3-(dimethylamino)phenyl ester
  • 16088-19-0
  • EINECS 240-240-5


HPLC Method for Analysis of Neostigmine methylsulfate on Primesep 100 Column


Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Neostigmine methylsulfate


Column Promesep 100, 4.6×150 mm,  100A
Mobile Phase MeCN – 30%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 210 nm


Class of Compounds Drug
Analyzing Compounds Neostigmine methylsulfate


Application Analytes:
Neostigmine methylsulfate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.

Primesep 100

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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Separation of Norneostigmine on Newcrom R1 HPLC column

Separation of Norneostigmine on Newcrom R1 HPLC column

Norneostigmine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Analytes:
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: or by phone: 847-229-2629.

Newcrom R1

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

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