Neostigmine methylsulfate

Neostigmine methylsulfate structural formula

CAS Number51-60-5
Molecular FormulaC13H22N2O6S
Molecular Weight334.391
InChI KeyOSZNNLWOYWAHSS-UHFFFAOYSA-M
LogP-0.961
Synonyms
  • Neostigmine methylsulfate
  • 3-[(Dimethylcarbamoyl)oxy]-N,N,N-trimethylanilinium methyl sulfate
  • 51-60-5
  • Ammonium, (3-(dimethylcarbamoyloxy)phenyl)trimethyl-, methylsulfate
  • Ammonium, (m-hydroxyphenyl)trimethyl-, methyl sulfate, dimethylcarbamate
  • Carbamic acid, N,N-dimethyl-, 3-dimethylaminophenyl ester, methosulfate
  • Carbamic acid, dimethyl-, ester with (m-hydroxyphenyl)trimethylammonium methyl sulfate
  • Carbamic acid, N,N-dimethyl-, 3-(trimethylammonio)phenyl ester, methylsulfate
  • Dimethylcarbamic ester of 3-oxyphenyltrimethylammonium methylsulfate
  • 3-(Dimethylcarbamoxy)phenyl trimethylammonium methyl sulfate
  • (3-(Dimethylcarbamoxy)phenyl)trimethylammonium methyl sulfate
  • (3-(Dimethylcarbamoyl)oxyphenyl)trimethylammonium methyl sulfate
  • EINECS 200-109-5
  • Eustigmin methyl sulfate
  • Eustigmin methylsulfate
  • Hodostin
  • (m-Hydroxyphenyl)trimethylammonium methyl sulfate dimethylcarbamate
  • (3-Hydroxyphenyl)trimethylammonium methyl sulfate dimethylcarbamic ester
  • Kirkstigmine methyl sulfate
  • Leostigmine methyl sulfate
  • Neoeserine methyl sulfate
  • Neostigmeth
  • Neostigmine methosulfate
  • Neostigmine methyl sulfate
  • Neostigmine monomethyl sulfate
  • Neostigmine monomethylsulfate
  • Normastigmin
  • NSC 93753
  • Philostigmin methyl sulfate
  • Polstigmine
  • Proserine methyl sulfate
  • Stigmanol methyl sulfate
  • Stigmosan methyl sulfate
  • Synstigmine
  • Synthostigmine methyl sulfate
  • Syntostigmin
  • Syntostigmin methyl sulfate
  • Syntostigmine methyl sulfate
  • Vagostigmine methyl sulfate
  • Proserine
  • Neostigminmetilsulfat
  • Proserinum
  • UNII-98IMH7M386
  • Bloxiverz
  • Prostigmin (TN)

Applications:

HPLC Method for Analysis of Neostigmine methylsulfate on Primesep 100 Column

May 11, 2022

HPLC Method for Neostigmine methylsulfate, Norneostigmine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Neostigmine methylsulfate, Norneostigmine.

Neostigmine methylsulfate is a drug with the chemical formula C13H22N2O6S. It is primarily used to reverse effects of non-depolarizing neuromuscular blocking agents after surgery. It works through inhibiting the enzyme acetylcholinesterase, preventing the breakdown of the neurotransmitter acetylcholine, which leads to a buildup of it at nerve endings, which stimulates muscle contractions.

Neostigmine methylsulfate, Norneostigmine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 30%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 210 nm

Description
Class of Compounds Drug
Analyzing Compounds Neostigmine methylsulfate, Norneostigmine

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Neostigmine methylsulfate
Norneostigmine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Neostigmine methylsulfate on Newcrom R1 HPLC column

May 16, 2018
Separation of Neostigmine methylsulfate on Newcrom R1 HPLC column

Neostigmine methylsulfate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Neostigmine methylsulfate
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.