| CAS Number | 28789-35-7 |
|---|---|
| Molecular Formula | C17H27NO3 |
| Molecular Weight | 293.408 |
| InChI Key | VQEONGKQWIFHMN-UHFFFAOYSA-N |
| LogP | 3.05 |
| Synonyms |
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Applications:
HPLC Separation of Nordihydrocapsaicin, Capsaicin and Dihydrocapsaicin
February 17, 2021
HPLC Method for Capsaicin, Dihydrocapsaicin, Nordihydrocapsaicin on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Capsaicin, Dihydrocapsaicin, Nordihydrocapsaicin.
Capsaicinoids (capsaicin, dihydrocapsaicin, and nordihydrocapsaicin) are a group of phenolic alkaloids specific to the genus Capsicum and are comprised of a vanillylamine head and a fatty acid tail.
Capsaicin, also known as 8-methyl-N-vanillyl-6-nonenamide, is an irritant with the chemical formula C18H27NO3. It found primarily in chili peppers. It is an irritant for mammals, producing a burning sensation in any tissue that exposed to it through touch. It is used primarily in cooking in the form of spices. Pharmacologically, it is used as an analgesic in topical ointments and dermal patches. It is also used in pepper spray and as a deterrent to pests.
Dihydrocapsaicin is a capsaicinoid and analog with the chemical formula C18H29NO3. It has the same pungency as capsaicin, while accounting for 22% of the total capsaicinoids mixture. It is soluble in dimethyl sulfoxide and 100% ethanol.
Nordihydrocapsaicin is a capsaicinoid and analog with the chemical formula C17H27NO3. It is less pungent than capsaicin. It makes up only 7% of total capsaicinoids mixture. On the Scoville scale it has 9,100,000 SHU (Scoville heat units).
Capsaicin, Dihydrocapsaicin, Nordihydrocapsaicin can be retained and separated in HPLC using Newcrom R1 reverse-phase column isocratically. The method uses a simple MS-compatible mobile phase of acetonitrile (ACN) and water without the need for a buffer. The peaks can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | No |
| Flow Rate | 0.5 ml/min |
| Detection | 220 nm |
| Class of Compounds | Hydrophobic |
| Analyzing Compounds | Capsaicin, Dihydrocapsaicin, Nordihydrocapsaicin |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Dihydrocapsaicin
Nordihydrocapsaicin
Separation of Nordihydrocapsaicin on Newcrom R1 HPLC column
February 16, 2018
Nordihydrocapsaicin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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