| CAS Number | 19408-84-5 |
|---|---|
| Molecular Formula | C18H29NO3 |
| Molecular Weight | 307.434 |
| InChI Key | XJQPQKLURWNAAH-UHFFFAOYSA-N |
| LogP | 3.76 |
| Synonyms |
|
Applications:
HPLC Separation of Nordihydrocapsaicin, Capsaicin and Dihydrocapsaicin
February 17, 2021
Separation type: Liquid Chromatography Reverse Phase
High Performance Liquid Chromatography (HPLC) Method of Nordihydrocapsaicin Capsaicin and Dihydrocapsaicin.
Capsaicinoids (capsaicin, dihydrocapsaicin and nordihydrocapsaicin) are a group of phenolic alkaloids specific to the genus Capsicum and are comprised of a vanillylamine head and a fatty acid tail. Capsaicin, dihydrocapsaicin and nordihydrocapsaic can be retained and separated in HPLC using Newcrom R1 reverse-phase column isocratically. The method uses a simple MS-compatible mobile phase of acetonitrile (ACN) and water without the need for a buffer. The peaks can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
| Column | Newcrom R1, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | No |
| Flow Rate | 0.5 ml/min |
| Detection | 220 nm |
| Class of Compounds | Hydrophobic |
| Analyzing Compounds | Nordihydrocapsaicin, Capsaicin, Dihydrocapsaicin |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsDihydrocapsaicin
Nordihydrocapsaicin
Separation of Dihydrocapsaicin on Newcrom R1 HPLC column
February 16, 2018
Dihydrocapsaicin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options