Separation type: Liquid Chromatography Reverse Phase
High Performance Liquid Chromatography (HPLC) Method of Nordihydrocapsaicin Capsaicin and Dihydrocapsaicin.
Capsaicinoids (capsaicin, dihydrocapsaicin and nordihydrocapsaicin) are a group of phenolic alkaloids specific to the genus Capsicum and are comprised of a vanillylamine head and a fatty acid tail. Capsaicin, dihydrocapsaicin and nordihydrocapsaic can be retained and separated in HPLC using Newcrom R1 reverse-phase column isocratically. The method uses a simple MS-compatible mobile phase of acetonitrile (ACN) and water without the need for a buffer. The peaks can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
| Column | Newcrom R1, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | No |
| Flow Rate | 0.5 ml/min |
| Detection | 220 nm |
| Class of Compounds | Hydrophobic |
| Analyzing Compounds | Nordihydrocapsaicin, Capsaicin, Dihydrocapsaicin |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsDihydrocapsaicin
Nordihydrocapsaicin
