| CAS Number | 51481-60-8 |
|---|---|
| Molecular Formula | C19H26ClNO6 |
| Molecular Weight | 399.870 |
| InChI Key | TXMZWEASFRBVKY-IOQDSZRYSA-N |
| LogP | 1.41 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Buprenorphine and Naloxone in Sublingual Film 8mg/2 mg on Primesep 100 Column
December 9, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of Buprenorphine and Naloxone on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Buprenorphine is an opioid partial agonist used to treat Opioid Use Disorder (OUD). Naloxone is used to treat the effects of opioids as part of a treatment for opiate addictions. When placed together in a sublingual film, they can be quite effective at treating opiate/narcotic addictions non-addictively. Buprenorphine and Naloxone can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 280 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Buprenorphine and Naloxone
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV,280 nm |
| Peak Retention Time | 3.8, 8.2 min |
Description
| Class of Compounds | Drugs |
| Analyzing Compounds | Buprenorphine, Naloxone |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Buprenorphine hydrochloride
Naloxone
Naloxone chlorhydrate
Separation of Naloxone chlorhydrate on Newcrom R1 HPLC column
May 17, 2018
Naloxone chlorhydrate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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