Buprenorphine hydrochloride

Buprenorphine hydrochloride structural formula

CAS Number53152-21-9
Molecular FormulaC29H42ClNO4
Molecular Weight504.110
InChI KeyUAIXRPCCYXNJMQ-RZIPZOSSSA-N
LogP3.94
Synonyms
  • Buprenorphine hydrochloride
  • (5alpha,6beta,14beta,18R)-17-(Cyclopropylmethyl)-18-[(2S)-2-hydroxy-3,3-dimethylbutan-2-yl]-6-methoxy-18,19-dihydro-4,5-epoxy-6,14-ethenomorphinan-3-ol--hydrogen chloride (1/1)
  • 6,14-Ethenomorphinan-3-ol, 17-(cyclopropylmethyl)-4,5-epoxy-18,19-dihydro-18-[(1S)-1-hydroxy-1,2,2-trimethylpropyl]-6-methoxy-, (5alpha,6beta,14beta,18R)-, hydrochloride (1:1)
  • 53152-21-9
  • Buprenex
  • EINECS 258-396-8
  • Finibron
  • Buprenorphine HCl
  • UNII-56W8MW3EN1
  • (-)-buprenorphine hydrochloride
  • (5alpha,6beta,14beta,18R)-17-(cyclopropylmethyl)-3-hydroxy-18-[(2S)-2-hydroxy-3,3-dimethylbutan-2-yl]-6-methoxy-18,19-dihydro-4,5-epoxy-6,14-ethenomorphinan-17-ium chloride
  • 17-cyclopropylmethyl-4,5alpha-epoxy-7alpha-((S)-1-hydroxy-1,2,2-trimethylpropyl)-6-methoxy-6,14-endo-ethanomorphinan-3-ol hydrochloride
  • 2-(N-cyclopropylmethyl-4,5alpha-epoxy-3-hydroxy-6-methoxy-6,14-endo-ethanomorphinan-6alpha-yl)-3,3-dimethyl-2-butanol hydrochloride
  • 21-cyclopropyl-7alpha-[(S)-1-hydroxy-1,2,2-trimethylpropyl]-6,14-endo-ethano-6,7,8,14-tetrahydrooripavine hydrochloride
  • buprenorphine HCl
  • buprenorphine hydrochlorie

Applications:

HPLC Method for Analysis of Buprenorphine and Naloxone in Sublingual Film  8mg/2 mg on Primesep 100 Column

December 9, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Buprenorphine and Naloxone on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Buprenorphine and Naloxone in Sublingual Film  8mg/2 mg on Primesep 100 Column
HPLC Method for Analysis of Buprenorphine and Naloxone on Primesep 100 by SIELC Technologies

Buprenorphine is an opioid partial agonist used to treat Opioid Use Disorder (OUD). Naloxone is used to treat the effects of opioids as part of a treatment for opiate addictions. When placed together in a sublingual film, they can be quite effective at treating opiate/narcotic addictions non-addictively. Buprenorphine and Naloxone can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 280 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Buprenorphine and Naloxone

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV,280 nm
Peak Retention Time3.8, 8.2 min

Description

Class of CompoundsDrugs
Analyzing CompoundsBuprenorphine, Naloxone

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Buprenorphine
Buprenorphine hydrochloride
Naloxone
Naloxone chlorhydrate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Buprenorphine hydrochloride on Newcrom R1 HPLC column

February 16, 2018
Separation of Buprenorphine hydrochloride on Newcrom C18 HPLC column

Buprenorphine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Buprenorphine hydrochloride
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.