| CAS Number | 616-91-1 |
|---|---|
| Molecular Formula | C5H9NO3S |
| Molecular Weight | 163.190 |
| InChI Key | PWKSKIMOESPYIA-BYPYZUCNSA-N |
| LogP | -0.695 |
| Synonyms |
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Applications:
Determination N-Acetyl-L-Cysteine on Primesep SB Column
February 25, 2021
HPLC Method for N-Acetyl-L-cysteine on Primesep SB by SIELC Technologies
| Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 20% |
| Buffer | H3PO4 0.3% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 50% |
| Buffer | Na2HPO4 pH 4.0 – 50 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
High Performance Liquid Chromatography (HPLC) Method for Analysis of N-Acetyl-L-cysteine.
N-Acetyl-L-Cysteine (NAC), also known as Acetylcysteine, is the N-acetyl derivative of the naturally occurring amino acid L-cysteine with the chemical formula C5H9NO3S. It is primarily used to treat paracetamol overdoses, chronci bronchopulmonary disorders, and lactobezoar.
N-Acetyl-L-cysteine can be retained on Primesep SB, a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 200 nm.
| Class of Compounds |
Nutritional supplement, Hydrophilic, Ionizable |
| Analyzing Compounds | N-Acetyl-L-cysteine |
Application Column
Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of N-Acetyl-L-cysteine on Newcrom R1 HPLC column
February 16, 2018
N-Acetyl-L-cysteine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options